Cryopreservation at BabyGen IVF (Freezing Eggs, Sperm and Embryos)

Cryopreservation

Cryopreservation at BabyGen IVF (Freezing Eggs, Sperm and Embryos)

What is cryopreservation?

Cryopreservation means cooling cells (eggs, sperm, embryos) to ultra‑low temperatures using liquid nitrogen, so all biological activity almost stops but the cells remain alive. With current vitrification technology, eggs and embryos can be frozen and thawed with high survival rates, making cryopreservation a safe and reliable part of fertility treatment.

Cryopreservation is the modern technique of freezing eggs, sperm and embryos at very low temperatures so they can be safely stored and used later for pregnancy. It allows couples to preserve their fertility, plan pregnancies at the right time, and get more than one chance from a single IVF or ICSI cycle. At BabyGen IVF, cryopreservation is a routine part of treatment, especially because we follow a “freeze‑all” approach for embryos in most cases before doing transfer.

Types of cryopreservation at BabyGen IVF

Embryo freezing

Embryo freezing is the most commonly used form of cryopreservation in IVF/ICSI cycles.
Fertilised eggs are grown in the lab for a few days and then the good‑quality embryos are frozen for transfer in a future cycle.

Egg (oocyte) freezing

Egg freezing allows women to preserve their eggs at a younger age or before treatments that might damage fertility (like chemotherapy or certain surgeries). These frozen eggs can later be thawed, fertilised and used to try for pregnancy when the woman is ready.

Sperm freezing

Sperm freezing is useful when the male partner cannot be available on the day of egg retrieval, has borderline semen parameters, or needs to preserve fertility before medical treatment. Frozen sperm can be used later for IUI, IVF or ICSI.

Our “freeze‑all” embryo protocol

At BabyGen IVF, in almost all IVF/ICSI cases we follow a freeze‑all strategy instead of doing a fresh transfer in the same cycle.

Why we freeze embryos in most cases
  • The woman’s body is recovering from stimulation injections, which can temporarily affect the uterine lining.
  • Freezing embryos allows hormone levels to settle and the uterus to be prepared calmly and optimally in a separate cycle.
  • This often improves implantation chances and reduces the risk of complications like OHSS.
When we freeze embryos

After IVF or ICSI, embryos are cultured in the lab and assessed on day 3, day 4 and, in some cases, day 5 (blastocyst stage).

Good‑quality embryos are frozen on day 3 or day 4 in most cases, according to our protocol, and stored safely until the transfer cycle planned approximately two months later.

How embryo cryopreservation works

Step 1 – Embryo development

Following egg retrieval and fertilisation (IVF or ICSI), embryos are grown in special incubators that maintain strict temperature, gas and pH control. Embryos are monitored for cell division, symmetry, fragmentation and overall quality.

Step 2 – Selection of embryos for freezing

Based on standard grading, good‑quality embryos on day 3 or day 4 are chosen for freezing. Any surplus suitable embryos beyond those intended for transfer in future cycles can also be frozen, giving you more chances from one stimulation cycle.

Step 3 – Vitrification (rapid freezing)

We use vitrification, a rapid‑freezing technique that turns the embryo’s cellular fluid into a glass‑like state without ice crystal formation. Embryos are first exposed to special cryoprotectant solutions and then quickly cooled in liquid nitrogen at around –196°C.

Step 4 – Storage in liquid nitrogen tanks

Frozen embryos are stored in specially designed liquid nitrogen tanks with monitored temperature and secure labelling. Each sample is carefully tagged so there is no mix‑up and can be tracked at every stage.

Step 5 – Thawing and transfer after two months

Around two months after freezing, we plan a frozen embryo transfer (FET) cycle. On the day of transfer, the selected embryos are gently warmed (thawed), washed free of cryoprotectants, and assessed for survival and quality before transfer to the uterus.

Benefits of cryopreservation for couples

Cryopreservation offers several advantages:

  • More than one chance from a single stimulation cycle.
  • Avoids repeated ovarian stimulation in many cases.
  • Allows safer, planned frozen embryo transfers in a relaxed cycle.
  • Protects fertility before treatments like chemotherapy.
  • Supports family planning and spacing of pregnancies.

For many couples, knowing that good‑quality embryos are safely frozen provides psychological comfort and a sense of security.

Is cryopreservation right for you?

Not every patient needs all forms of cryopreservation, but most IVF/ICSI patients benefit from embryo freezing. Egg or sperm freezing may be particularly important if you are at risk of losing fertility or want to preserve options for the future.

Your fertility specialist will discuss what to freeze, how many to freeze, storage duration and expected outcomes based on your age and clinical situation.

Safety and success of cryopreservation

Cryopreservation with modern vitrification has very high survival rates for embryos and good results for eggs and sperm. Worldwide, millions of healthy babies have been born from frozen embryos and frozen gametes, and freezing is now considered a standard part of fertility care. 

While no method can guarantee pregnancy, cryopreservation gives you more opportunities from each cycle and flexibility in planning your family.

How long can we store frozen embryos, eggs and sperm?

In practice, embryos, eggs and sperm can be stored for many years as long as the storage conditions remain stable and regulations are followed.

Legally allowed storage duration may vary by country and local guidelines, and you will be asked to sign consent forms for storage period and future use.

Why choose BabyGen IVF for cryopreservation?

At BabyGen IVF, we integrate cryopreservation into routine care with:

  • A dedicated embryology team trained in vitrification.
  • Strict lab protocols for labelling, storage and monitoring.
  • A  freeze‑all embryo protocol with planned transfer after about two months.

This combination of technology and protocol helps maximise the utility of every egg and embryo you produce.

Book your cryopreservation consultation

If you are planning IVF/ICSI, considering delaying pregnancy, or facing a medical treatment that might affect fertility, a consultation on cryopreservation can clarify your options.